A Large Scale Procedure for Isolation of the Glycine-rich, Arginine-rich Histone and the Arginine-rich, Lysine-rich Histone in a Highly Purified Form

A large scale procedure for isolation of the glycine-rich, arginine-rich histone and the arginine-rich, lysine-rich histone in a highly purified form.

1. A procedure is presented for large scale fractionation of arginine-rich histones by exclusion chromatography. With this method, 2 to 3.5 g of histone Fractions 2a and 3 were fractionated. The two fractions contained the same histone components, but they differed in the amounts of each component. 2. In histone Fraction 2a, the glycine-rich, arginine-rich (GAR) histone accounted for approximat...

Structure of the glycine-rich, arginine-rich histone of the Novikoff hepatoma.

Structural analysis of the glycine-rich, arginine-rich histone. 3. Sequence of the amino-terminal half of the molecule containing the modified lysine residues and the total sequence.

1. The amino acid sequence of the amino-terminal portion of the glycine-rich, arginine-rich histone (Residues 1 to 49) has been determined from studies on the tryptic, chymotryptic, and thermolysin peptides. This sequence overlaps with that of the carboxylterminal portion (Residues 50 to 102) defined previously to give the following sequence: acetylserGly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys...

A Method for Differential Colour Staining of Lysine-Rich and Arginine-Rich Histones in Polyacrylamide Gel

K, and V,,,,,. values for ethanolamine, 1 -aminopropan-2-01s and their phosphate derivatives, with the kinase and phospho-lyase enzymes respectively, indicated that the pathway in Pseudomonas sp. N.C.I.B. 8858 was concerned with 1-aminopropan-2-01 degradation. (L-1-Aminopropan-2-01 is a metabolite of aminoacetone, derived from L-threonine, and D-1-aminopropan-2-01 is a fragment of vitamin B12 c...

Immunochemical specificity in lysine-rich histone subfractions.

Antibodies to purified calf thymus Fl, and to chromatographically purified subfractions of calf thymus Fl histones were induced by immunization of rabbits with histone-RNA complexes. Complement fixation studies with these antibodies demonstrated structural differences between the subfractions in one organ and between those of different organs and species. The anti-F1 sera showed varying reactiv...